First off, I love Twi like this. As said before by BratB2084, kitty pony style is present. Fur, blush and expression look very lifelike and totally adorable
Second: the mane shading technique is just perfect, with a watercolor brush-like shade, and without the colors in her mane having contours, like in MLP FIM
And the third: Twi's expression. I'd say your style makes ponies cuter than ever, especially Twi. The expression is so humanlike, so realistic that you can just see Twi's sassiness (and cuteness) in full view She's just like a baby kitty pony. Great job and Stay AWSOME!